A 505

c-d-Ala

++++ +++

++++ ++

c-d-Val

++++ +++

+++ ++

c-d-Leu

++++ ++++

++++ +++

c-d-Phe

++++ +++

+++ ++

c-d-PEG

+++ +++

++ +

c-d-PG

+++ +++

+ - + -

c-d-HPG

+++ +++

+ + +-

Note: With 1 mL culture broth of each isolated strain, the resting cell reaction was performed under standard conditions at 30 °C (mesophile, 3-h reaction) or 45 °C (thermophile, 24-h reaction), followed by analysis by TLC. Seven N-carbamoyl-D-amino acids (c-D-amino acids) were examined, and the amount of D-amino acid produced in the reaction is represented approximately as follows: +- , 0.1 g/L; + , 0.2 g/L; ++ , 0.5 g/L; + + + , 1-2 g/L; + + + +, 4 g/L. PEG and PG represent phenetylglycine and phenyl-glycine, respectively.

Note: With 1 mL culture broth of each isolated strain, the resting cell reaction was performed under standard conditions at 30 °C (mesophile, 3-h reaction) or 45 °C (thermophile, 24-h reaction), followed by analysis by TLC. Seven N-carbamoyl-D-amino acids (c-D-amino acids) were examined, and the amount of D-amino acid produced in the reaction is represented approximately as follows: +- , 0.1 g/L; + , 0.2 g/L; ++ , 0.5 g/L; + + + , 1-2 g/L; + + + +, 4 g/L. PEG and PG represent phenetylglycine and phenyl-glycine, respectively.

Table 2. Properties of DCases from Agrobacterium sp. KNK712 and Pseudomonas sp. KNK003A

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