Corynebacterium glutamicum, which produces L-gluta-mate, requires biotin for growth but excessively forms L-glutamic acid at a biotin concentration suboptimal for growth. Under the condition of excess biotin, L-glutamic acid can be produced by (1) adding penicillin (19) or a surface-active agent (20), or (2) culturing an oleic acid-requiring strain at growth-limiting oleic acid concentrations (20,21). Cane molasses, beet molasses, and corn syrup are mainly used as the carbon source because of their low cost. By way of example, L-glutamic acid fermentation by the fed-batch method using cane molasses is described here. Although cane molasses contains most of the
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