Aspartame Production by Thermolysin

The principles of the thermolysin process were introduced by Oyama et al. (9) in 1987. At first, L-PheOMe from racemic PheOMe is coupled with Z-Asp stereo and regio selectively by thermolysin. The reaction product, Z-APM, forms an insoluble addition compound with one molecule of remaining D-PheOMe immediately and precipitates. When the reaction is completed, the Z-APM-D-PheOMe addition compound is collected as a solid product, and ther-molysin is recovered from the solution. D-PheOMe is separated from Z-APM as its hydrochloride by acid washing (with hydrochloric acid, for example). The Z-group is removed from the Z-APM by hydrogenolysis. Finally, APM is obtained by crystallization and drying. D-PheOMe is recycled after racemization by alkaline treatment and rees-terification in methanol. The process is summarized in Figure 2.

Brew Your Own Beer

Brew Your Own Beer

Discover How To Become Your Own Brew Master, With Brew Your Own Beer. It takes more than a recipe to make a great beer. Just using the right ingredients doesn't mean your beer will taste like it was meant to. Most of the time it’s the way a beer is made and served that makes it either an exceptional beer or one that gets dumped into the nearest flower pot.

Get My Free Ebook


Post a comment