B

Figure 2. Effect of ammonia on murine hybridoma culture. (a) Cell growth and death rates, and (b) maximum viable and total cell concentrations in batch culture. Source: Data obtained from Ozturk and Palsson (88).

Thus, the reduction in cell density at higher ammonia concentrations was simply the result of lower cell specific growth rates. On the other hand, Goergen et al. (89) used much higher concentrations of ammonia (up to 17 mM) and observed an increase in the death rate. Newland et al. (80) also reported the influence on death rates by ammonia at high concentrations. More quantitative studies are needed to verify the effects of ammonia on specific death rates and the mechanism(s) involved.

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