A number of studies reported the effects of ammonia on cell metabolism. For a murine hybridoma cell line, Ozturk et al. (6) observed a roughly twofold increase in glucose and glutamine consumption rates in cultures exposed to 3.75 mM of ammonia, compared to control conditions (Fig. 3). The production rates of lactate and ammonia were also enhanced by the presence of ammonia, indicating an elevated metabolic state of the cells. The yield coefficients of ammonia from glutamine and lactate yield from glucose decreased about 15% with 3.75 mM ammonia (Fig. 3). A similar elevation in metabolic rates was reported by Miller et al. (52) for AB2-143.2 hybridoma cells and by Alex et al. (90) forC127 cells. Glacken (87) has also shown an increase in the glutamine consumption rate by added ammonia.
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