Fermentation broth

Spent and washing

Lysine fractions

Spent and washing

Neutralization with HCl

Neutralization with HCl

2nd concentration

2nd concentration

Product: L-lysine HCl salt (feed-grade)

Mother liquor —Absorption on resin

Product: L-lysine HCl salt (feed-grade)

Figure 12. Purification process flow of l-lysine HCl salt (feed-grade).

combinants of aromatic amino acids biosynthetic genes in E. coli are used. The direct fermentation method using mutants and recombinants and the enzymatic method are currently practiced. Because L-tryptophan is not highly soluble in the aqueous media, the purification methods have been developed to take this into consideration.

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