Each of the techniques we described has advantages and drawbacks. When designing experiments to identify and quantify apoptosis, a number ofpoints should be taken into consideration. First, there can be significant variations in the morphology of apoptosis from one cell type to the next, including, for example, absence of chromatin condensation. It is therefore recommended that several methods be used simultaneously to identify the mechanism of cell death. However, quantification of actual levels of apoptosis can vary significantly depending upon the technique used. Thus, in order to draw valid conclusions, it is essential that comparison of quantitative data collected using one technique is not made with data collected using a second technique.
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