Hooc

COOMe

Scheme 6.

PheOMe proceeds spontaneously. Fumaric-PheOMe was reportedly produced in good yield by isomerization ofMPM (40). Unfortunately, the yield at the step of ammonia addition is too low to be applicable in an industrial APM production process. A new microbial strain exhibiting higher lyase activity has recently been reported by Xu et al. (43), but even with this strain, productivity seems to be insufficient. Further screening of bacteria and improvement of ammonia lyase thus appears to be important.

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