and product formation could be studied in the second CSTR.

Adding an additional feedstream to the second stage (Fig. 6) provides the opportunity to introduce more of the limiting nutrient, other nutrients required for growth or product formation, inducers, or inhibitors. The material balances on cell mass and substrate on the second stage can be written as equations 53 and 54, respectively, with steady-state solutions given in Table 5. In equation 53, F is the volumetric flow of the second feedstream, which is assumed to be sterile (X = 0).

Figure 6. Two-stage chemostat system with possibility of a separate feed to second reactor.

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