Info

Figure 8. Nucleotide sequence of the adp gene and the predicted amino acid sequence. In this open reading frame of 1,164 bp (388 amino acids), shown with the deduced amino acid sequence, sequences confirmed by the protein sequencer are underlined.

Bacillus cereus ADP (48) 55

Streptomyces Cpase DD (30) 83

Streptomyces lactamdurans PBP (49) 50

Bacillus subtilis PBP 4 (50) 50

Serratia marcescens BLA (51) 69

Enterobacter cloacae BLA (52) 74

Dichelobacter nodosus Protein D (53) 103

Ochrobactrum anthropi DAP (28) 55

Pseudomonas sp. Esterase (31) 65

MKTDFRFR IG S VT K TFTATVV 75

I TTTDRFR VG S VT K SFSAVVL 103

VTTDSVFQ SG S VA K VYTATLV 70

LKTNSLFE LA S LS K PFTALGI 70

I TEQTLFE VG S LS K TFTATLA 89

VTPQTLFE LG S IS K TFTGVLG 94

MRVNSRFR YA S VT K VLTSALV 123

MTLDTRMP IC S VS K QFTCAVL 75

WHSDTIVN LF S CT K TFTAVTA 85

Figure 9. Partial alignments of the amino acid sequences of ADP and other similar enzymes. Met55 found in the partial amino acid sequence of ADP corresponds to 387ATG in Figure 8; abbreviations as in Figure 4.

Table 4. Comparison of Properties of the D-Amino Acid-Containing Peptide Hydrolases

Property

d-Aminopeptidase

Alkaline d-peptidase

Origin

Ochrobactrum anthropi

Bacillus cereus

Molecular weight

114,514 (57,252 X 2)

37,952

Number of subunits

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