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Thermostable temperature is defined as the temperature on heat treatment for 10 min that caused a decrease in the DCase activity of 50%.

Thermostable temperature is defined as the temperature on heat treatment for 10 min that caused a decrease in the DCase activity of 50%.

bation at 40 °C for 4 h, and then the residual activity was measured under standard conditions. The relative activity compared with the activity of each nontreated sample was plotted against the incubation pH (Fig. 6). The stability of the mutant enzyme from KNK455M was increased in the lower- and higher-pH regions in comparison with those of the native enzyme.

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