carbon, nitrogen, phosphate, and minerals for microbial growth, its use as a carbon source is basic, and the growth medium is optimized by supplementing with other nutrient sources. Such medium contains an excess concentration of biotin. Potassium salt of penicillin G (8 units/L) is added to the culture broth late in the logarithmic growth phase. Upon addition of penicillin, the microorganism produces L-glutamic acid—mainly due to a change of the permeating system and/or the enzyme activities in TCA cycle (22)—and L-glutamic acid is excreted into the broth. Fermentation is continued while feeding molasses and con-
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