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carbon, nitrogen, phosphate, and minerals for microbial growth, its use as a carbon source is basic, and the growth medium is optimized by supplementing with other nutrient sources. Such medium contains an excess concentration of biotin. Potassium salt of penicillin G (8 units/L) is added to the culture broth late in the logarithmic growth phase. Upon addition of penicillin, the microorganism produces L-glutamic acid—mainly due to a change of the permeating system and/or the enzyme activities in TCA cycle (22)—and L-glutamic acid is excreted into the broth. Fermentation is continued while feeding molasses and con-

Fermentation broth

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Brew Your Own Beer

Brew Your Own Beer

Discover How To Become Your Own Brew Master, With Brew Your Own Beer. It takes more than a recipe to make a great beer. Just using the right ingredients doesn't mean your beer will taste like it was meant to. Most of the time it’s the way a beer is made and served that makes it either an exceptional beer or one that gets dumped into the nearest flower pot.

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