Figure 2. Synthesis of d-alanine from alanine amide HCl by the cells of E. coli JM109/pC138DP. The reaction mixture contained various amounts of racemic alanine amide HCl, washed E. coli cells harvested from 2.0 mL of culture broth, and 50 imol of tris(HCl), pH 8.0, in a total volume of 2.0 mL. The enzyme activity shown by the E. coli transformant was 288,000 Units per liter of culture. The mixtures were incubated at 30 °C. Formation of d-alanine from d-alanine amide is shown as yield (%).
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