Figure 6. Effect of pH on the enzyme stability of the mutagenized DCases. The mutant enzyme from KNK455M was incubated at 40 °C for 4 h at the indicated pH in 0.1 M K2HPO4-KH2PO4 (solid line), 0.1 M Tris-HCl (dashed line), or Na2CO3-NaHCO3 (dotted line) buffer. The remaining activities were then assayed under standard conditions and expressed as a percentage of the activity of each nontreated enzyme. As a control, the native enzyme (asterisk) was treated in the same manner.

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Brew Your Own Beer

Brew Your Own Beer

Discover How To Become Your Own Brew Master, With Brew Your Own Beer. It takes more than a recipe to make a great beer. Just using the right ingredients doesn't mean your beer will taste like it was meant to. Most of the time it’s the way a beer is made and served that makes it either an exceptional beer or one that gets dumped into the nearest flower pot.

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