Figure 6. Effect of pH on the enzyme stability of the mutagenized DCases. The mutant enzyme from KNK455M was incubated at 40 °C for 4 h at the indicated pH in 0.1 M K2HPO4-KH2PO4 (solid line), 0.1 M Tris-HCl (dashed line), or Na2CO3-NaHCO3 (dotted line) buffer. The remaining activities were then assayed under standard conditions and expressed as a percentage of the activity of each nontreated enzyme. As a control, the native enzyme (asterisk) was treated in the same manner.
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