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Figure 4. Time course of the condensation reaction of Z-l-Asp and l-PheOMe catalyzed by 150 mg of wild type (closed circle) and 150 mg (closed triangle) and 50 mg (open circle) of TZ-1, respectively, in 200 mL of a reaction mixture containing 0.2 M of Z-l-Asp 0.5 M of d,l-PheOMe, 5.1 mM CaCl2, and 0.17 MNaCl at pH 6.0 at 40 °C.

Table 2. Condensation Reaction at Different Z-Aspartic Acid-to-PheOMe Ratio

Figure 4. Time course of the condensation reaction of Z-l-Asp and l-PheOMe catalyzed by 150 mg of wild type (closed circle) and 150 mg (closed triangle) and 50 mg (open circle) of TZ-1, respectively, in 200 mL of a reaction mixture containing 0.2 M of Z-l-Asp 0.5 M of d,l-PheOMe, 5.1 mM CaCl2, and 0.17 MNaCl at pH 6.0 at 40 °C.

(32). This mutant is called TZ-5 and will be useful for further reducing of costs in APM production.

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