Info

Note: The mutagenized DCase genes were analyzed and the amino acid substitutions resulting from the mutations were deduced. To compare the thermostability of the mutant enzymes easily, the index of thermostability, thermostable temperature, was defined as the temperature on heat treatment for 10 min that caused a decrease in DCase activity of 50%. The substituted amino acids, on random mutagenesis, in these mutants are indicated by asterisks.

Note: The mutagenized DCase genes were analyzed and the amino acid substitutions resulting from the mutations were deduced. To compare the thermostability of the mutant enzymes easily, the index of thermostability, thermostable temperature, was defined as the temperature on heat treatment for 10 min that caused a decrease in DCase activity of 50%. The substituted amino acids, on random mutagenesis, in these mutants are indicated by asterisks.

Table 5. Thermostability of Mutant DCases Having Two or Three Mutations

Strain

Thermostable temperature® (°C)

Amino acid mutation

57 His

203 Pro

236 Val

Strain

Thermostable temperature® (°C)

57 His

203 Pro

236 Val

Brew Your Own Beer

Brew Your Own Beer

Discover How To Become Your Own Brew Master, With Brew Your Own Beer. It takes more than a recipe to make a great beer. Just using the right ingredients doesn't mean your beer will taste like it was meant to. Most of the time it’s the way a beer is made and served that makes it either an exceptional beer or one that gets dumped into the nearest flower pot.

Get My Free Ebook


Post a comment