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amino acids and related compounds. Like the native enzyme, the enzyme showed strict specificity toward N-carbamoyl-D-amino acids, N-carbamoyl-L-amino acids not being hydrolyzed. N-Carbamoyl-D-amino acids having a hydrophobic side chain, as well as N-carbamoyl-amino acids having a polar group or short-chain alkyl group, were hydrolyzed. The substrate specificity of the mutant enzyme was almost the same as that of the native enzyme (Table 6). It seemed that these three thermostability-related amino acid changes little affected the substrate specificity. The other known DCases from Agrobacterium showed relatively similar tendencies as to substrate specificity.

Brew Your Own Beer

Brew Your Own Beer

Discover How To Become Your Own Brew Master, With Brew Your Own Beer. It takes more than a recipe to make a great beer. Just using the right ingredients doesn't mean your beer will taste like it was meant to. Most of the time it’s the way a beer is made and served that makes it either an exceptional beer or one that gets dumped into the nearest flower pot.

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