The design of a process for the large-scale purification of biological molecules is one of the several complex tasks that must be successfully completed to bring a product to market. Most downstream processes include some chromatography steps as well as other conventional separation methods. Chromatography is generally recognized as the technique that enables the highest degree of purification of biomolecules. For example, the U.S. Food and Drug Administration (FDA) recommends that at least two chromatography steps be used in the purification of therapeutic-grade monoclonal antibodies.

Chromatography separations are sophisticated and can be expensive processes, which explains the continuous development of effective approaches by scientists and engineers to improve the productivity of chromatography technologies. Fluidized bed adsorption, various column technologies, computerized optimization strategies, and continuous and semicontinuous processes are some of the recent approaches that have been proposed. However, one of the most important aspects of chromatography technology is the choice of matrix.

in this article, we examine the use of inorganic rigid matrices in chromatography separation processes of biological molecules, with emphasis on the preparation, properties, and applications of controlled pore glass (CPG).

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