Knk003a

Molecular weight (monomer) SDS-PAGE 37,000 38,000

DNA sequence 34,300 35,400

Thermal stability (60 °C, 20 min)

(residual activity) 5% 95%

Optimal temperature 67 °C

Number of amino acids (deduced)

Total 304 312

Hydrophobic 162 154

Neutral 50 51

Hydrophilic 92 107

Cystein 5 5

Homology

DNA 62%

Amino acid 60%

Sma I

Sma I

Figure 1. Restriction maps of pAD108 and pPD304. The thin line indicates the vector DNA and the boxed line the inserted DNA fragment. The open reading frame is represented from ATG to term (the black-boxed line).

102 AMINOHYDROLASES, FOR PRODUCTION OF d-AMINO ACIDS (a)

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