Molecular weight (monomer) SDS-PAGE 37,000 38,000
DNA sequence 34,300 35,400
Thermal stability (60 °C, 20 min)
(residual activity) 5% 95%
Optimal temperature 67 °C
Number of amino acids (deduced)
Total 304 312
Hydrophobic 162 154
Neutral 50 51
Hydrophilic 92 107
Cystein 5 5
Amino acid 60%
Figure 1. Restriction maps of pAD108 and pPD304. The thin line indicates the vector DNA and the boxed line the inserted DNA fragment. The open reading frame is represented from ATG to term (the black-boxed line).
102 AMINOHYDROLASES, FOR PRODUCTION OF d-AMINO ACIDS (a)
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