Outline

Introduction

Aspartame Production by Protease

Advantages of Aspartame Production by Protease

Aspartame Production by Thermolysin

Improvement of the Condensation Reaction

Stability of Enzymes

Immobilized Thermolysin

Enzyme Improvement by Site-Directed Mutagenesis

Evaluation of Mutant Thermolysins in APM Production

Stabilization of Thermolysin by Change of Reaction Conditions

Alternative Processes

Condensation without N-Protection

Production from Other Raw Materials

Conclusion

Acknowledgments

Bibliography

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