Figure 6. Effect of pH on the enzyme stability of the mutagenized DCases. The mutant enzyme from KNK455M was incubated at 40 °C for 4 h at the indicated pH in 0.1 M K2HPO4-KH2PO4 (solid line), 0.1 M Tris-HCl (dashed line), or Na2CO3-NaHCO3 (dotted line) buffer. The remaining activities were then assayed under standard conditions and expressed as a percentage of the activity of each nontreated enzyme. As a control, the native enzyme (asterisk) was treated in the same manner.

Brew Your Own Beer

Brew Your Own Beer

Discover How To Become Your Own Brew Master, With Brew Your Own Beer. It takes more than a recipe to make a great beer. Just using the right ingredients doesn't mean your beer will taste like it was meant to. Most of the time it’s the way a beer is made and served that makes it either an exceptional beer or one that gets dumped into the nearest flower pot.

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