Q22

Source: Glacken (49).

Source: Glacken (49).

ammonia yield was reported to be between 0.5 to 1 mol of ammonia generated per mole of glutamine consumed (6,33,50-52). Glutamine concentrations in typical cell culture media range between 1 and 7 mM (17). Thus, the ammonia concentrations in cell culture can reach 5 mM (6,5053). Consequently, the higher glutamine concentrations found in special media formulations yield much more ammonia levels. Glutamine concentrations used in fed-batch cultures, for instance, can vary between 8 and 40 mM, and very high ammonia concentrations are obtained (14,54,55).

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