Stability of the Immobilized Enzyme

For repeated batch reactions with the immobilized DCase, a substrate solution (3%) of c-D-HPG (pH 7.0) was prepared, with nitrogen gas bubbling at 40 °C. The DCase immobilized on Duolite A-568 and the prepared substrate

Figure 7. Construction profile of pNT4553.

Ampr pNT4553

Ampr pNT4553

solution were stirred at 40 °C under a stream of nitrogen gas while the pH was controlled at pH 7.0 with 2 M HCl. Samples were taken for activity measurement at 10 and 60 min, the reaction being continued for 23.5 h in total. The reaction mixture was removed by suction, after which a fresh substrate solution was introduced and allowed to react in the same manner as already described. The residual activity was measured as the relative activity compared with the initial activity and plotted against the reaction times (Fig. 8). The stability of the mutant en zyme increased in comparison with that of the native enzyme.

Brew Your Own Beer

Brew Your Own Beer

Discover How To Become Your Own Brew Master, With Brew Your Own Beer. It takes more than a recipe to make a great beer. Just using the right ingredients doesn't mean your beer will taste like it was meant to. Most of the time it’s the way a beer is made and served that makes it either an exceptional beer or one that gets dumped into the nearest flower pot.

Get My Free Ebook


Post a comment