Substrate Specificities of the DCases

The substrate specificity of the enzyme from KNK455M was investigated using a broad range of N-carbamoyl-D-

Figure 5. Effect of temperature on the stability of the mutagenized enzymes. A cell-free extract of each mutant DCase-producing strain was kept at various temperatures and the remaining activity was assayed under standard conditions. The remaining activities of the mutant enzymes from KNK402M (solidline, squares), KNK404M (dottedline, circles), KNK416M (solidline, triangles), and KNK455M (solidline, circles), and the native enzyme (asterisks), are expressed as a percentage of the activity of each non-heated enzyme.

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