Utilization Of The Improved Dcase For Industrial Production

An enzymatic conversion process involving the improved DCase in an immobilized form was introduced for d-HPG production instead of chemical diazonation. As a result of the introduction of the new process, the efficiency of the

Figure 8. Stability of the immobilized DCase on repeated batch reactions. Repeated batch reactions were carried out using the immobilized mutant DCase from KNK404M (circles) and the native enzyme (asterisks).

Number of reactions (times)

Figure 8. Stability of the immobilized DCase on repeated batch reactions. Repeated batch reactions were carried out using the immobilized mutant DCase from KNK404M (circles) and the native enzyme (asterisks).

14. Y. Ikenaka, H. Nanba, K. Yajima, Y. Yamada, M. Takano, and S. Takahashi, Biosci. Biotechnol. Biochem. 62, 1668-1671 (1998).

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reaction was greatly increased, the purification process became simpler because of the decrease in the undesirable color formation of the product, and the energy required for production was reduced. In addition to these advantages, by-products such as inorganic salts were greatly reduced by the change in the reaction mechanism. By means of these advantages, the new process decreases the burden on the environment.

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