Although different substrates can be used to produce tempeh, the basic fermentation process is similar for all substrates. It includes soaking, dehulling (if necessary), boiling and fermenting. The main differences between different substrates used in tempeh fermentation are the selection of optimal pre-treatments (e.g. when cereals are used it is sometimes necessary to modify the surface of the grain by cutting, cracking or pearling to obtain good growth of the mold), optimal soaking, boiling and incubating time, and optimal fermentation strain and inoculation level of R. oligosporus.
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