The acetone-formaldehyde separation would be an example of affinity difference chromatography in which molecules of similar molecular weight or isomers of compounds are separated on the basis of differing attractions or distribution coefficients for the resin. The largest industrial chromatography application of this type is the separation of fructose from glucose to produce 55% or 90% fructose corn sweetener.

Ion exclusion chromatography involves the separation of an ionic component from a nonionic component. The ionic component is excluded from the resin beads by ionic repulsion, while the nonionic component will be distributed into the liquid phase inside the resin beads. Since the ionic solute travels only in the interstitial volume, it will reach the end of the column before the nonionic solute which must travel a more tortuous path through the ion exchange beads. A major industrial chromatography application of this type is the recovery of sucrose from the ionic components of molasses.

In size exclusion chromatography, the resin beads act as molecular sieves, allowing the smaller molecules to enter the beads while the larger molecules are excluded. Figure 31181 shows the effect of molecular size on the elution volume required for a given resin. The ion exclusion technique has been used for the separation of monosodium glutamate from other neutral amino acids.1191

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