Uj

1 100

• LABORATOnr

• LABORATORY

• PRODUCTION

30 60 90 120 150 160 210 240 2)0 TIME IN MINUTES

Figure 22. Comparison of experimental curves for laboratory and production columns.!70'

Figure 22. Comparison of experimental curves for laboratory and production columns.!70'

Column Diameter (meter)

Figure 23. Scaleup relationships for fluidized beds.'711

5.5 Chromatographic Scale-Up Procedures

The aim of scaling-up a chromatographic process is to obtain the same yield and product quality in the same time period on laboratory, preparative and industrial scale. Laboratory analytical purifications tend to be optimized only for resolution of individual solutes, however, at the preparative and production scale, it is necessary also to maximize throughput. Table 14[73] shows the effect of chromatographic operating parameters on resolution and throughput. While column length is a critical factor for resolution with gel filtration and isocratic elutions, it has little effect on resolution with gradient elution in adsorption chromatography. The wall effects on resolution are very noticeable with small radius columns, but decrease as the column length is increased.

Table 14. Effects of Process Parameters on Resolution and Throughput'73'

Resolution Throughput

Parameter varies with varies with

Column Length (L)

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