Cell Disruption

Micro-organisms are protected by extremely tough cell walls. In order to release their cellular contents a number of methods for cell disintegration have been developed (Wimpenny, 1967; Hughes et al., 1971). Any potential method of disruption must ensure that labile materials are not denatured by the process or hydrol-ysed by enzymes present in the cell. Huang et al. (1991) report the use of a combination of different techniques to release products from specific locations within yeast cells. In this way the desired product can be obtained with minimum contamination. Although many techniques are available which are satisfactory at laboratory scale, only a limited number have been proved to be suitable for large-scale applications, particularly for intracellular enzyme extraction (Wang et al., 1979; Darbyshire, 1981). Containment of cells can be difficult or costly to achieve in many of the methods described below and thus containment requirements will strongly influence process choice. Methods available fall into two major categories:

Physico-mechanical methods

(a) Liquid shear.

(c) Agitation with abrasives.

(d) Freeze-thawing.

(e) Ultrasonication.

Physical-mechanical methods

Was this article helpful?

0 0
The Miracle Of Vinegar

The Miracle Of Vinegar

You may be forgiven for thinking that these passed down secrets had gone for good, washed away with time and the modern age, But they're not. You can now own three of the best traditional did you know style reports that were much loved by our parents and grandparents. And they were pretty smart too because not only will these reports save you time and money but they'll also help you eliminate some of the scourges of modern day living such as harmful chemical usage in the home.

Get My Free Ebook

Post a comment