I

Minimal medium

(Incubation with shaking for 22 h at 30°C Cell suspension (7 X 10s —1.1 X 109 cells cm-3)

I U.V. or 60Co-irradiation I (7 cm3/dish) Complete medium

(Incubation for 8 h washing of cells, 2-3 times Minimal medium plus 5-50ucm~3 penicillin

II ncubation for 12 -16 h Centrifugation

Cells | Plate out Complete agar

Incubation for 24 h at 30° C

Minimal agar Complete agar

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