(Incubation with shaking for 22 h at 30°C Cell suspension (7 X 10s —1.1 X 109 cells cm-3)
I U.V. or 60Co-irradiation I (7 cm3/dish) Complete medium
(Incubation for 8 h washing of cells, 2-3 times Minimal medium plus 5-50ucm~3 penicillin
II ncubation for 12 -16 h Centrifugation
Cells | Plate out Complete agar
Incubation for 24 h at 30° C
Minimal agar Complete agar
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