Osmotic Shock

Osmotic shock caused by a sudden change in salt concentration will cause disruption of a number of cell types. However, the effect on microbial cells is normally minimal. It has proved to be a successful technique for the extraction of luciferase from Photobac-terium fischeri (Hastings et al., 1965). A batch of 120 dm3 of broth was harvested and the cells collected as a cell paste in a Sharpies centrifuge. Enzyme extraction was achieved by osmotic lysis using a ratio of 1 g of cell paste to 4 cm3 of cold distilled water with stirring for

Fig. 10.22. Simplified drawing of the Dyno-Muhle KD5 (Mogren et al., 1974). (1) inlet of suspension; (2) manometer; (3) rotating disc; (4) slit for separation of glass beads from the suspension; (5) outlet of suspension; (6) thermometer; (7) cooling water, inlet and outlet; (8) bearings; (9) variable V-belt drive; (10) drive motor. Cylinder dimensions: inside length 33 cm; inside diameter 14 cm.

Fig. 10.22. Simplified drawing of the Dyno-Muhle KD5 (Mogren et al., 1974). (1) inlet of suspension; (2) manometer; (3) rotating disc; (4) slit for separation of glass beads from the suspension; (5) outlet of suspension; (6) thermometer; (7) cooling water, inlet and outlet; (8) bearings; (9) variable V-belt drive; (10) drive motor. Cylinder dimensions: inside length 33 cm; inside diameter 14 cm.

15 to 30 minutes. A second extraction gave a small additional yield of enzyme. Only low levels of soluble protein were released using this technique.

Vinegar For Your Health

Vinegar For Your Health

A resource for the many ways you can use Vinegar to improve your health! In today's society of miracle medicine, we often overlook things that have been around hundreds of years! Things like Vinegar!

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