Process

Regardless of the type of fermentation (with the possible exception of some transformation processes) an established process may be divided into six basic component parts:

(i) The formulation of media to be used in cultur-ing the process organism during the development of the inoculum and in the production fermenter.

(ii) The sterilization of the medium, fermenters and ancillary equipment.

(iii) The production of an active, pure culture in sufficient quantity to inoculate the production vessel.

(iv) The growth of the organism in the production fermenter under optimum conditions for product formation.

(v) The extraction of the product and its purification.

(vi) The disposal of effluents produced by the process.

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