Screening methods

Early screening strategies tended to be empirical, labour intensive and had relatively low success rates. As the number of commercially important compounds isolated increased, the success rates of such screens decreased further. Thus, new screening methods have been developed which are more precisely targeted to identify the desired activity. The evolution of antibiotic

Table 3.3 Selective substrates for the isolation of actinomycetes and antibiotic-producing actinomycetes

Substrates selective for Actinomycetes

Substrates selective for antibiotic-producting actinomycetes

Proline

Proline

Glucose (1.0%)

Glucose (1.0%)

Glycerol

Glycerol

Starch

Starch

Humic acid (0.1%)

Humic acid (0.1%)

Propionate (0.1%)

Zinc

Methanol

Alanine

Nitrate

Potassium

Calcium

Vitamins

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