Solid Shear

Pressure extrusion of frozen micro-organisms at around -25°C through a small orifice is a well established technique at a laboratory scale using a Hughes press or an X-press to obtain small samples of enzymes or microbial cell walls. Disruption is due to a combination of liquid shear through a narrow orifice and the presence of ice crystals. Magnusson and Edebo (1976) developed a semi-continuous X-press operating with a sample temperature of - 35°C and an X-press temperature of - 20°C. It was possible to obtain 90% disruption with a single passage of S. cerevisiae using a throughput of 10 kg yeast cell paste h"1. This technique might be ideal for microbial products which are very temperature labile.

Was this article helpful?

0 0
Brew Your Own Beer

Brew Your Own Beer

Discover How To Become Your Own Brew Master, With Brew Your Own Beer. It takes more than a recipe to make a great beer. Just using the right ingredients doesn't mean your beer will taste like it was meant to. Most of the time it’s the way a beer is made and served that makes it either an exceptional beer or one that gets dumped into the nearest flower pot.

Get My Free Ebook

Post a comment