The Preservation Of Industrially Important Microorganisms

The isolation of a suitable organism for a commercial process may be a long and very expensive procedure and it is therefore essential that it retains the desirable characteristics that led to its selection. Also, the culture used to initiate an industrial fermentation must be viable and free from contamination. Thus, industrial cultures must be stored in such way as to eliminate genetic change, protect against contamination and retain viability. An organism may be kept viable by repeated sub-culture into fresh medium, but, at each cell division, there is a small probability of mutations occurring and because repeated sub-culture involves very many such divisions, there is a high probability that strain degeneration would occur. Also, repeated sub-culture carries with it the risk of contamination. Thus, preservation techniques have been developed to maintain cultures in a state of 'suspended animation' by storing either at reduced temperature or in a dehydrated form. Full details of the techniques are given by Kirsop and Doyle (1991).

Storage at reduced temperature

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