Regarding the number of samples to be removed, a reasonably good characterization of the kinetics will typically be achieved with around 10 sampling times, chosen to include the whole growth curve, with many of these taken during the rapid growth phase (Fig. 15.4). The best interval between samples will then depend on how fast the organism grows, and is not necessarily uniform. For a fast-growing organism, it may be appropriate to remove samples every two hours for twenty hours after the lag phase. For a slower grower, a sampling interval of 6 hours to 1 day, or even longer, might be appropriate. It would be desirable to remove triplicate samples at each sampling time; however, the number of replicates will depend on the resources available. With Raimbault columns, it is not uncommon for workers to remove only a single column at each sampling time.

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